Optimization of protein buffer cocktails using Thermofluor

The Thermofluor assay constitutes a quick and easy-to-perform high-throughput method with which it is possible to identify protein-stabilizing buffer compositions or small-molecule additives.

Cloning, expression, purification and preliminary X-ray analysis of the dimerization domain of ethylene response sensor 1 (ERS1) from Arabidopsis thaliana

The central dimerization domain of the ethylene receptor ERS1 from A. thaliana was expressed, purified and crystallized. The crystals belonged to space groups C222₁ and P2₁2₁2.

Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana

The catalytic domain of the histidine kinase of the ethylene receptor ETR1 from A. thaliana was expressed and crystallized. The crystals belonged to space group I222 or I2₁2₁2₁.