Optimization of protein buffer cocktails using Thermofluor

The Thermofluor assay constitutes a quick and easy-to-perform high-throughput method with which it is possible to identify protein-stabilizing buffer compositions or small-molecule additives.

Cloning, expression, purification and preliminary X-ray analysis of the dimerization domain of ethylene response sensor 1 (ERS1) from Arabidopsis thaliana

The central dimerization domain of the ethylene receptor ERS1 from A. thaliana was expressed, purified and crystallized. The crystals belonged to space groups C222₁ and P2₁2₁2.