The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein

J. L. Watkins, H. Kim, M. L. Markwardt, L. Chen, R. Fromme, M. A. Rizzo and R. M. Wachter

The high resolution X-ray structure of the cyan fluorescent protein mCerulean3 demonstrates that different combinations of correlated residue substitutions can provide near optimum quantum yield values for fluorescence.

Keywords: GFP-like proteins; enhanced cyan fluorescent proteins; ECFP; chromophores; directed evolution; structure-guided protein engineering; FRET; quantum yield.

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