research papers
A systematic approach by the molecular-replacement method with a dimeric search model using the program Phaser led to an unexpected pentameric structure of the NSP4:95–146 region of the ST3 strain of rotavirus, in contrast to the previously reported tetramers.
Keywords: nonstructural proteins; NSP4; rotaviruses; enterotoxins; diarrhoea; molecular replacement.
research papers
DEN refinement and automated model building with AutoBuild were used to determine the structure of a putative succinyl-diaminopimelate desuccinylase from C. glutamicum. This difficult case of molecular-replacement phasing shows that the synergism between DEN refinement and AutoBuild outperforms standard refinement protocols.
research papers
A density-based procedure is described for improving a homology model that is locally accurate but differs globally. The model is deformed to match the map and refined, yielding an improved starting point for density modification and further model-building.
research papers
An account of the detection of severe diffraction anisotropy, rotational pseudosymmetry and twinning during the refinement of the rotaviral nonstructural protein NSP4 and a description of corrections for these factors leading to successful refinement of the structure are presented.