Insights into the binding specificity and catalytic mechanism of N-acetylhexosamine 1-phosphate kinases through multiple reaction complexes

The seven crystal structures presented in this work represent the first multiple reaction complexes (GlcNAc/GalNAc, GlcNAc-1P/GalNAc-1P, ATP/ADP and Mg²⁺) of NahK in the lacto-N-biose (LNB)/galacto-N-biose (GNB) pathway, providing the molecular basis for the recognition of substrates and gaining insights into the catalytic mechanism at the molecular level.

Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase

The first structural complexes of the ferric ion-SAM-dependent C-methyltransferase MppJ are reported, whereon MppJ was engineered performing unforeseen Lewis acid-assisted hydration and/or O-methyltransfer reactions to form novel stereospecific compounds.