Celebrating biological crystallography: Acta D twenty years on

Editorial.

Placement of molecules in (not out of) the cell

The importance of presenting macromolecular structures in unified, standard ways is discussed.

Expectation bias and information content

Editorial.

On optimal placement of molecules in the unit cell

Standard ways for the placement of molecules in the unit cell are proposed.

Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis

The yellow fluorescent protein phiYFPv with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium.

On the reproducibility of protein crystal structures: five atomic resolution structures of trypsin

Details of five very high-resolution accurate structures of bovine trypsin are compared in the context of the reproducibility of models obtained from crystals grown under identical conditions.

Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine

The crystal structure of the novel red emitting fluorescent protein from lancelet Branchiostoma lanceolatum (Chordata) revealed an unusual five residues cyclic unit comprising Gly58-Tyr59-Gly60 chromophore, the following Phe61 and Tyr62 covalently bound to chromophore Tyr59.