Changing of the guard

DNA and beyond

Purification, crystallization and preliminary X-ray analysis of Escherichia coli UDP-N-acetylmuramoyl:L-alanine ligase (MurC)

MurC is responsible for the first step in the addition of the pentapeptide unit of peptidoglycan. Native and SeMet crystals of the E. coli enzyme have been prepared.

Acta D ten years on

Making the most of two crystals: structural analysis of a conserved hypothetical protein using native gel screening and SAD phasing

A protein of unknown function, PAE2307, from the hyperthermophilic archaeon P. aerophilum has been crystallized and gel screening has been used to identify a suitable derivative for SAD phasing, given only two usable crystals.

Crystallization and preliminary X-ray analysis of N-acetyl-1-D-myo-inosityl-2-deoxy-α-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis

The deacetylase MshB from M. tuberculosis (Rv1170), which is involved in the biosynthesis of the key antioxidant mycothiol, has been expressed, purified and crystallized in a form suitable for high-resolution X-ray structural analysis.