Acta Crystallographica Section D
Acta Crystallographica
Section D
STRUCTURAL BIOLOGY
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Acta Cryst.
(2013).
D
69
,
991-996
https://doi.org/10.1107/S0907444913004046
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Structure and functional studies of the ribonuclease binase Glu43Ala/Phe81Ala mutant
V. A. Mitkevich
,
A. A. Schulga
,
A. A. Trofimov
,
P. V. Dorovatovskii
,
D. A. Goncharuk
,
E. N. Tkach
,
A. A. Makarov
and
K. M. Polyakov
The Glu43Ala/Phe81Ala mutation in binase prevents dimerization of the enzyme and improves its catalytic activity and cytotoxicity.
Keywords:
ribonucleases
;
binase
;
site-directed mutagenesis
;
enzymatic activity
;
cytotoxicity
.
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