Gd-HPDO3A, a complex to obtain high-phasing-power heavy-atom derivatives for SAD and MAD experiments: results with tetragonal hen egg-white lysozyme

A neutral gadolinium complex, Gd-HPDO3A, is shown to be a good candidate to obtain heavy-atom derivatives and solve macromolecular structures using anomalous dispersion. Tetragonal crystals of a gadolinium derivative of hen egg-white lysozyme were obtained by co-crystallization using different concentrations of the complex. Diffraction data from three derivative crystals were collected to a resolution of 1.7 Å using Cu Kα radiation from a rotating anode.

Detection and characterization of merohedral twinning in two protein crystals: bacteriorhodopsin and p67^phox

A combination of various approaches was used to determine precise twinning ratios. The importance of second-, third-, and fourth-order moments is discussed.

FIP: a highly automated beamline for multiwavelength anomalous diffraction experiments

FIP is a French CRG beamline at the European Synchrotron Radiation Facility (ESRF) dedicated exclusively to crystallography of biological macromolecules, with a special emphasis on automated multiwavelength anomalous diffraction data collection in the 0.7-1.81 Å wavelength range.