Stealth carriers for low-resolution structure determination of membrane proteins in solution

Specifically deuterated phospholipid bilayer nanodiscs have been developed which give a minimal contribution to neutron scattering data when used in 100% D₂O. These provide an optimal platform for low-resolution structural determination of membrane proteins and their complexes in solution.

Multi-lamellar organization of fully deuterated lipid extracts of yeast membranes

This first structural characterization of fully deuterated natural lipid extracts has revealed that the membrane stack is complex and regulated mainly by electrostatic repulsion between adjacent membranes. Structural differences with respect to hydrogenous analogues are investigated as a function of temperature and humidity.

Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography

The advantages of perdeuteration with regard to solvent visibility in macromolecules is demonstrated through the comparison of two neutron protein structures.