A branched N-linked glycan at atomic resolution in the 1.12 Å structure of rhamnogalacturonan acetylesterase

The structure of the glycoprotein rhamnogalacturonan acetylesterase has been refined at 1.12 Å resolution. The branched N-linked glycan structure has been refined without target values for bond lengths and angles and the resulting values are compared with the current dictionary values obtained from small-molecule studies.

Crystallization and preliminary X-ray characterization of a thermostable pectate lyase from Thermotoga maritima

The successful crystallization and characterization by X-ray diffraction of a recombinant mutant pectate lyase from the hyperthermophilic bacterium T. maritima is described.

Structure of product-bound Bacillus caldolyticus uracil phosphoribosyltransferase confirms ordered sequential substrate binding

The structure of uracil phosphoribosyltransferase from B. caldolyticus with bound UMP is described and a catalytic mechanism is proposed.

A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus

The stepwise optimization of crystallization conditions, characterization by X-ray diffraction and space-group changes upon heavy-atom derivatization of a rhamnogalacturonan lyase from A. aculeatus are reported.