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In the title compound, C22H27N3O9S, the six-membered pyranosyl ring adopts a chair conformation. The acetyl group opposite the thiosemicarbazone substituent occupies an axial position, while all other substituents are in equatorial positions. The mol­ecules are linked by C—H...O hydrogen bonds into chains parallel to the b axis, and two additional C—H...O inter­actions provide further stability in a three-dimensional network.
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