Acta Crystallographica Section D
Acta Crystallographica
Section D
STRUCTURAL BIOLOGY
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Acta Cryst.
(2005).
D
61
,
799-802
https://doi.org/10.1107/S0907444905008085
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Development of a novel ampholyte buffer for isoelectric focusing: electric charge-separation of protein samples for X-ray crystallography using free-flow isoelectric focusing
S.-H. Kim
,
H. Miyatake
,
T. Ueno
,
T. Nagao
and
K. Miki
A novel ampholyte buffer suitable for crystallization using the ProTeam FFE apparatus has been developed. This buffer is able to generate pH gradients owing to the use of low-molecular-weight electrolytes.
Keywords:
free-flow electrophoresis
;
charge separation
;
ampholyte buffer
.
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