Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD⁺

The crystal structure of NAD⁺-bound FDH from P. aeruginosa was determined to 2.7 Å resolution. The overall structure is very similar to that of P. putida FDH. Preliminary kinetics analysis of the enzyme indicated a glutathione-independent bi-substrate `ping-pong' catalytic mechanism.

Crystallization and preliminary X-ray diffraction analysis of MJ0458, an adenylate kinase from Methanocaldococcus jannaschii

MJ0458 protein, an adenylate kinase from Methanocaldococcus jannaschii which is a rich resource of unique enzymes, was crystallized and a set of X-ray diffraction data to 2.70 Å resolution was collected on the beamline BL-17U of the Shanghai Synchrotron Radiation Facility (SSRF). The crystal belonged to space group P4₁2₁2 or P4₃2₁2. The unit-cell parameters were a = b = 76.18, c = 238.70 Å, α = β = γ = 90°.