Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method

A new semi-automated protein crystal mounting device has been developed for the capillary-top mounting method, which has advantages for the sulfur single-wavelength anomalous diffraction phasing method using longer-wavelength X-rays.

In-crystal chemical ligation for lead compound generation

A new fragment-based growth strategy (in-crystal chemical ligation) for lead compound generation is proposed, which is based on in situ chemical ligation and is operable in X-ray-based fragment screening format. Proof-of-concept experiments demonstrate that protein crystals charged with an anchor molecule not only function as a reaction field for chemical ligation but also serve as a platform on which to select self-assembled products.