Structure of D-3-hydroxybutyrate dehydrogenase prepared in the presence of the substrate D-3-hydroxybutyrate and NAD⁺

The crystal structure of A. faecalis D-3-hydroxybutyrate dehydrogenase prepared in the presence of D-3-hydroxybutyrate and NAD⁺ reveals the substrate/product-binding geometry as the first example which suggests that the catalytic reaction occurs by shuttle movements of a hydrogen negative ion from the substrate to NAD⁺ and from NADH to the product.

Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94

A novel haloalkane dehalogenase DbeA from B. elkani USDA94 and its mutant variant DbeA1 were crystallized and diffraction data were collected to 2.2 Å resolution.