Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases

Using lysozyme and Fc receptor crystals as test cases, it was demonstrated that conventional heavy-atom derivatives could be generated by quick soaking in a 10 min to 2 h time frame instead of days. Derivatives generated in this way showed stronger heavy-atom signals and appeared to be more isomorphous to the native than derivatives generated by overnight soaks.