Download citation
Download citation

link to html
Numerous examples show that with accurate diffraction data SAD phasing leads to a rapid solution of macromolecular crystal structures.

Download citation
Download citation

link to html
The structure of recombinant RNase Sa from 1.0 Å spacing synchrotron data collected at cryogenic temperature shows two alternative conformations of the main chain in two loops which contain active-site residues. This feature was not observed in the structure refined against room-temperature data at 1.2 Å resolution.

Download citation
Download citation

link to html
Comparison of the structure of the adenovirus proteinase, whose crystallization and X-ray diffraction are reported here, with that of the enzyme activated by the 11-amino acid peptide pVIc will reveal why the enzyme is inactive and how the enzyme is activated by pVIc. The low solvent content, 29.2%, may be the reason why this crystal diffracted to such high resolution, 0.98 Å.

Download citation
Download citation

link to html
SAD phasing may often be successful in cases intended for MAD, leading to structure solution before the second MAD-wavelength data collection is finished. The background of SAD phasing is presented using various vector diagrams.

Download citation
Download citation

link to html
The crystal structure of the bacterial catalase from M. lysodeikticus has been refined at 0.88 Å using the gene-derived sequence, revealing the active site with unprecedented accuracy. In addition, the structures of the ferryl intermediate of the catalase (compound II) at 1.96 Å resolution and the catalase complex with NADPH at 1.83 Å resolution have been determined.
Keywords: catalase.

Download citation
Download citation

link to html
Studies of a number of Glu→Ala single and multiple mutants of RhoGDI show that such mutations may be generally useful to enhance protein crystallizability. One of the mutants yielded data to 1.2 Å resolution, showing that surface mutants can be used to dramatically improve the resolution of X-ray data.
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds