Crystallization and preliminary crystallographic analysis of a metastasis-inducing protein, human S100A4

SeMet S100A4 (human) has been crystallized using similar conditions to those used for the native protein. SeMet S100A4 crystals diffract better than the native for data collection and belong to space group P6 or P3.

Application of direct methods to protein crystallographic phase extension

A new formulation of direct methods using the sign probability originally introduced for breaking the phase ambiguity has been proposed, in which the known phase information could be used as constrained phases in the phase refinement. This new formulation has been applied to protein crystallographic phase extension and shown to be effective.