Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519

E. Y. Bezsudnova, M. V. Kovalchuk, A. V. Mardanov, K. M. Poliakov, V. O. Popov, N. V. Ravin, K. G. Skryabin, V. A. Smagin, T. N. Stekhanova and T. V. Tikhonova

Recombinant ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and used to produce crystals that diffracted to 3.09 Å resolution.

Keywords: DNA ligases; archaea.

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