Crystallization and preliminary X-ray characterization of the 2,4'-dihydroxyaceto­phenone dioxygenase from Alcaligenes sp. 4HAP

The enzyme 2,4'-dihydroxyacetophenone dioxygenase (or DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. By the use of limited proteolysis, the DAD enzyme from Alcaligenes sp. 4HAP has been crystallized in a form that diffracts synchrotron radiation to a resolution of 2.2 Å.