Expression, purification and crystallization of a fungal type III polyketide synthase that produces the csypyrones

CsyB from A. oryzae has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.71 Å.

Crystallization and preliminary X-ray diffraction analysis of AntE, a crotonyl-CoA carboxylase/reductase from Streptomyces sp. NRRL 2288

AntE, a crotonyl-CoA carboxylase/reductase involved in the biosynthesis of antimycin A from Streptomyces sp. NRRL 2288, has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 2.29 Å.