Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia

PigI, a key enzyme in the biosynthesis of prodigiosin, catalyzes the first step by converting L-proline to L-prolyl-AMP. Here the crystallization and preliminary crystallographic analysis of PigI is reported.

Crystallization and preliminary X-ray diffraction analysis of Gos1p, a yeast SNARE protein

Gos1 protein (Golgi SNAP receptor complex member 1) is involved in the SNAREs complexes which are the core machinery driving membrane fusion between cargo-carrying vesicles and their target membranes in the secretory and endocytic pathways in yeast. In this paper, the crystallization method of truncated versions of the Gos1 protein from Saccharomyces cerevisiae is reported and preliminary data on its X-ray analysis are provided.