Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia

PigI, a key enzyme in the biosynthesis of prodigiosin, catalyzes the first step by converting L-proline to L-prolyl-AMP. Here the crystallization and preliminary crystallographic analysis of PigI is reported.

Crystallization and preliminary crystallographic analysis of defective pollen wall (DPW) protein from Oryza sativa

Mutations in the defective pollen wall (dpw) gene cause abnormalities in the development of anthers and pollen grains of O. sativa. In this study, DPWΔ80 protein was cloned and expressed in E. coli. Purified DPWΔ80 in complex with its cofactor NADPH formed crystals. Diffraction data were collected to 3.4 Å resolution.