Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

T. Furuyama, Y. Ishii, N. Ohya, K. Tateda, N. D. Hanson and A. Shimizu-Ibuka

The class B carbapenemase IMP-18 from P. aeruginosa was overexpressed in E. coli, purified and crystallized. The crystals belonged to space group P4₁2₁2 and diffracted to 2 Å resolution.

Keywords: class B β-lactamases; IMP-18; carbapenemases; Pseudomonas aeruginosa.

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