crystallization communications
ADH2, an NAD(P)-dependent butanol dehydrogenase A, is part of the terminal oxidation pathway in the long-chain alkane-degrading G. thermodenitrificans NG80-2. The expression, purification, crystallization and preliminary crystallographic analysis of ADH2 are reported.
structural communications
The high-resolution crystal structure of human Dim2/TXNL4B indicated that a region previously reported to be responsible for its interaction with Prp6 is unlikely to facilitate this interaction.
crystallization communications
Purification, crystallization and preliminary crystallographic analysis of the marine α-amylase AmyP
A new marine derived α-amylase AmyP, classified into a new subfamily of GH13, was recombinantly expressed, crystallized and preliminarily analyzed.
structural communications
The crystal structure of NAD+-bound FDH from P. aeruginosa was determined to 2.7 Å resolution. The overall structure is very similar to that of P. putida FDH. Preliminary kinetics analysis of the enzyme indicated a glutathione-independent bi-substrate `ping-pong' catalytic mechanism.
structural communications
The conserved protein MO25 activates STE20 family kinases. The crystal structure of zebrafish MO25 is reported, which is similar to that of human MO25.