Purification, crystallization and preliminary X-ray crystallographic analysis of the CIDE-N domain of Fsp27

X. Wang, B. Zhang, D. Xu, J. Gao, L. Wang, Z. Wang, Y. Shan and X. Yu

The CIDE-N domain of Fsp27 expressed in E. coli was purified as a monomer and identified by LC/MS/MS. Crystals of the Fsp27 CIDE-N domain were grown in sitting-drop mode and diffracted to 1.92 Å resolution.

Keywords: CIDEC; Fsp27; obesity; prokaryotic expression; unilocular lipid droplets.

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