Expression, purification, crystallization and preliminary X-ray crystallographic analysis of L-­lactate dehydrogenase and its H171C mutant from Bacillus subtilis

Recombinant wild-type L-lactate dehydrogenase from B. subtilis (BsLDH) was cocrystallized with fructose 1,6-bisphosphate and NAD⁺ and the crystal diffracted to 2.38 Å resolution. The H171C mutant of BsLDH was also crystallized as the apoenzyme and in complex with NAD⁺ and the crystals diffracted to 2.20 and 2.49 Å, respectively. All crystals belonged to space group P3.