Perdeuteration, purification, crystallization and preliminary neutron diffraction of an ocean pout type III antifreeze protein

I. Petit-Haertlein, M. P. Blakeley, E. Howard, I. Hazemann, A. Mitschler, M. Haertlein and A. Podjarny

Perdeuterated type III antifreeze protein has been expressed, purified and crystallized. Preliminary neutron data collection showed diffraction to 1.85 Å resolution from a 0.13 mm³ crystal.

Keywords: type III antifreeze proteins; neutron diffraction; perdeuteration.

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