Crystallization, characterization and preliminary X-ray crystallographic analysis of GK2848, a putative carbonic anhydrase of Geobacillus kaustophilus

The expression, purification, characterization and crystallization of GK2848, a carbonic anhydrase from G. kaustophilus, are described. The crystals diffracted to a resolution of 2.70 Å.

Crystallization and preliminary X-ray crystallographic analyses of Thermus thermophilus backtracked RNA polymerase

Backtracked bacterial RNA polymerase (RNAP) complexes were reconstituted by assembling Thermus thermophilus RNAP with nucleic acid scaffolds. The complexes were crystallized, and their X-ray diffraction data were collected and analyzed.

Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426

The gene encoding the hypothetical DUF1811-family protein GK0453 from G. kaustophilus was cloned and expressed. The crystal structure of the protein was determined by the molecular-replacement method and was refined to 2.2 Å resolution.