Improved crystallization of Escherichia coli ATP synthase catalytic complex (F₁) by introducing a phosphomimetic mutation in subunit [epsilon]

A phosphomimetic mutation in subunit [epsilon] dramatically increases reproducibility for crystallization of Escherichia coli ATP synthase catalytic complex (F₁) (subunit composition α₃β₃γ[epsilon]). Diffraction data were collected to ∼3.15 Å resolution using synchrotron radiation.