research papers
Crystal structures with and without His-tags have been surveyed and the authors show that His-tags are not hazardous to your structure.
research papers
The vaccinia virus dUTPase structure shows an increased size of the central channel within the homotrimer.
research papers
The C-terminal His tag is resolved in the structures of the apoenzyme and complexes with AMP and pyrophosphate or a substrate analog of ATP, wherein it is found to interact with native residues at the dimer interface. In the complex structures, it partially occupies the native binding site for the substrate NaAD.