High-syn conformation of uridine and asymmetry of the hexameric molecule revealed in the high-resolution structures of Shewanella oneidensis MR-1 uridine phosphorylase in the free form and in complex with uridine

The three-dimensional structures of uridine phosphorylase from S. oneidensis MR-1 in the free form and in complex with uridine were determined at atomic resolution (0.95 Å) and high resolution (1.6 Å), respectively. The uridine molecule has an energetically unfavourable high-syn conformation with a nearly planar ribose ring. The hexamer of uridine phosphorylase was found to be asymmetric.