Split green fluorescent protein as a modular binding partner for protein crystallization

H. B. Nguyen, L.-W. Hung, T. O. Yeates, T. C. Terwilliger and G. S. Waldo

A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants.

Keywords: protein crystallization; synthetic symmetrization; protein tagging; split GFP; split protein; green fluorescent protein; protein expression; protein-fragment complementation; crystallization reagents.

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