Improving experimental phases for strong reflections prior to density modification

A genetic algorithm has been developed to optimize the phases of the strongest reflections in SIR/SAD data. This is shown to facilitate density modification and model building in several test cases.

Model morphing and sequence assignment after molecular replacement

A procedure for model building is described that combines morphing a model to match a density map, trimming the morphed model and aligning the model to a sequence.

Split green fluorescent protein as a modular binding partner for protein crystallization

A strategy using a new split green fluorescent protein (GFP) as a modular binding partner to form stable protein complexes with a target protein is presented. The modular split GFP may open the way to rapidly creating crystallization variants.