Bulk-solvent and overall scaling revisited: faster calculations, improved results

A fast analytical method for calculating mask-based bulk-solvent scale factors and overall anisotropic correction factors is introduced.

Structure of the protein core of translation initiation factor 2 in apo, GTP-bound and GDP-bound forms

The crystal structures of the eubacterial translation initiation factor 2 in apo form and with bound GDP and GTP reveal conformational changes upon nucleotide binding and hydrolysis, notably of the catalytically important histidine in the switch II region.

On effective and optical resolutions of diffraction data sets

The notion of `resolution' is discussed and a formal definition applicable to various diffraction data sets, both complete and incomplete and both isotropic and anisotropic, is suggested.