Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase

The first structural complexes of the ferric ion-SAM-dependent C-methyltransferase MppJ are reported, whereon MppJ was engineered performing unforeseen Lewis acid-assisted hydration and/or O-methyltransfer reactions to form novel stereospecific compounds.