Divalent metal ion-based catalytic mechanism of the Nudix hydrolase Orf153 (YmfB) from Escherichia coli

The structures of Escherichia coli YmfB on its own and in complex with three sulfates and two manganese ions, when compared with the structures of other Nudix hydrolases such as MutT, Ap₄Aase and DR1025, provide insight into the unique hydrolysis mechanism of YmfB.

Structural basis for the recognition of muramyltripeptide by Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the helical cell shape

H. pylori Csd4 (HP1075), together with other peptidoglycan hydrolases, plays an important role in determining the helical cell shape. Its crystal structure has been determined in three different forms.