Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila

T. Siddiqui, J. J. Paxman, C. Dogovski, S. Panjikar and M. A. Perugini

Dihydrodipicolinate synthase (DHDPS) catalyses the rate-limiting step in the biosynthesis of meso-diaminopimelate and lysine. Here, the cloning, expression, purification and crystallization of DHDPS from the intracellular pathogen Legionella pneumophila are described.

Keywords: dihydrodipicolinate synthase; Legionella pneumophila.

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