Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana Deg8

Crystals of Deg8, an ATP-independent serine endopeptidase from A. thaliana, were monoclinic, belonging to space group C2 with unit-cell parameters a = 129.5, b = 124.2, c = 93.3 Å, β = 132.4°, and diffracted to 2.0 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of EtFPOX from Eupenicillium terrenum sp.

Crystals of EtFPOX from E. terrenum sp. diffracted to 1.9 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a = 65.6, b = 80.0, c = 83.4 Å.

Expression, purification and preliminary X-ray crystallographic analysis of Arf1-GDP in complex with dimeric p23 peptide

The complex of Arf1-GDP and dimeric p23 peptide, which is likely to be involved in membrane binding of Arf1, has been crystallized and preliminary crystallographic analysis was performed.

Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein

The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. cAMP-free D53H CRP crystals were obtained and diffracted at a resolution of 2.9 Å. Based on the systematic absences of the crystals, the likely space group is P2₁2₁2₁ with the unit-cell parameters a = 76.66, b = 152.14, and c = 176.11 Å. The asymmetric unit was confirmed to contain four protein dimers with a Matthews coefficient of 2.71 Å³ Da^-1 and a solvent content of 54.68%.