Crystallization and preliminary X-ray characterization of a type III cohesin-dockerin complex from the cellulosome system of Ruminococcus flavefaciens

O. Salama-Alber, Y. Gat, R. Lamed, L. J. W. Shimon, E. A. Bayer and F. Frolow

The cloning, expression, purification, crystallization and preliminary X-ray characterization of a trimodular type III cohesin-dockerin complex from the R. flavefaciens cellulosome system are described.

Keywords: scaffoldins; X modules; selenomethionine; single-wavelength anomalous diffraction.

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