Co-crystallization of the human nuclear cap-binding complex with a m⁷GpppG cap analogue using protein engineering

A long solvent-exposed coiled coil in the large subunit (CBP80) of the heterodimeric nuclear cap-binding complex (CBC) has been truncated to permit crystallization of CBC with the cap analogue m⁷GpppG in two crystal forms diffracting to 2.15 and 2.3 Å resolution.