Crystallization of a stringent response factor from Aquifex aeolicus

Mutations and deliberate oxidation of the protein have been used to enhance the possibilities of experimental phasing. The variant protein crystallized under different conditions to the wild-type protein and produced a crystal form of increased physical stability.

Structural analysis of the two horseradish peroxidase catalytic residue variants H42E and R38S/H42E: implications for the catalytic cycle

The crystal structures of two mutants of horseradish peroxidase C (HRPC) with substitutions of the key catalytic residues Arg83 and His42 have been determined to 2.5 Å (H42E HRPC) and 2.0 Å (R38S/H42E HRPC) resolution.