Crystallization and X-ray analysis of native and selenomethionyl β-mannanase Man5A from blue mussel, Mytilus edulis, expressed in Pichia pastoris

About 40% incorporation of SeMet in Man5A β-mannanase from blue mussel, M. edulis, was obtained with the commercial P. pastoris expression system. The presence of selenium in the crystals was confirmed by an X-ray fluorescence scan. Diffraction to 1.5 Å has been obtained.