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Dextranase has been expressed in P. pastoris with about 50% incorporation of selenomethionine. The enzyme has been crystallized in space group P21212, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Å and one molecule per asymmetric unit. The crystals diffract to 2.0 Å.
Keywords: dextranase.

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About 40% incorporation of SeMet in Man5A β-mannanase from blue mussel, M. edulis, was obtained with the commercial P. pastoris expression system. The presence of selenium in the crystals was confirmed by an X-ray fluorescence scan. Diffraction to 1.5 Å has been obtained.
Keywords: β-mannanase.
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