Crystallization and preliminary X-ray crystallographic studies on acyl-(acyl carrier protein) from Escherichia coli

Butyryl-ACP from E. coli, its selenomethionine (SeMet) variant and SeMet butyryl-ACP I62M have been crystallized. Crystals of the native protein and SeMet butyryl-ACP I62M belong to space group P2₁2₁2₁ and diffract to beyond 1.4 Å, whereas those of native SeMet butyryl-ACP belong to space group P6₃ and diffract to at least 2 Å.

Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius

Leucine dehydrogenase from T. intermedius has been crystallized as a binary complex with 2-ketoisocaproate and as a non-productive ternary complex with NAD⁺ and 2-ketoisocaproate in forms suitable for structure determination.

Crystallization and preliminary X-ray analysis of the ytxM gene product from Bacillus subtilis

The B. subtilis ytxM gene product has been crystallized and a MAD data set has been collected to 2.0 Å resolution.

Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin

Crystals of B. cepacia cholesterol oxidase, a 3β-hydroxysteroid oxidase containing covalently bound FAD, were obtained by the hanging-drop method using polyethylene glycol as a precipitant. The crystals diffract to beyond 2.0 Å and belong to space group P3₁21, with approximate unit-cell parameters a = b = 119.6, c = 101.1 Å and one subunit in the asymmetric unit.