Structure of starch synthase I from barley: insight into regulatory mechanisms of starch synthase activity

The crystal structure of starch synthase I from barley was refined to 2.7 Å resolution. It includes a regulatory disulfide and a bound oligosaccharide. Activity assays were performed on several mutants.

New features of vault architecture and dynamics revealed by novel refinement using the deformable elastic network approach

Here, the re-refinement of the structure of the vault particle by incorporating the high-resolution information available for the R1-7 domains, using the deformable elastic network (DEN) approach and maintaining strict 39-fold noncrystallographic symmetry is reported.