Atomic resolution structures of ribonuclease A at six pH values

Six structures of bovine pancreatic ribonuclease at six pH values have been refined and analyzed. A comparative analysis of the six models and some biological implications are discussed.

Crystallization of the collagen-like polypeptide (PPG)₁₀ aboard the International Space Station. 2. Comparison of crystal quality by X-ray diffraction

Crystals of the collagen-like polypeptide (PPG)₁₀ were obtained within the Advanced Protein Crystallization Facility on board the International Space Station, during the STS-105/STS-108 mission. The duration of this mission was such to ensure that the crystallization process had reached its end. Crystals were grown both in the presence and in the absence of agarose gel, to compare the quality of the crystals obtained from these different environments. As a result, crystals grown in the absence of agarose on Earth as well as in microgravity showed X-ray diffraction up to 1.15 Å. The intensity/sigma ratio was slightly higher for microgravity grown crystals. Crystals grown in agarose gel, both in microgravity and on ground, showed a comparable diffraction power, with a resolution limit of 1.45 Å.