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Acta Cryst. (2014). A70, C414
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During the biosynthesis of tail-anchored (TA) membrane proteins, their single C-terminal trans-membrane segment is inserted into the ER membrane for orientating the functional domain(s) towards the cytosolic side of the cell. The machinery responsible for this post-translational process has only recently come to light. In yeast, the proteins participating in TA protein insertion include Get1/Get2, Get3, Get4 and Get5. Although structural information and the individual roles of most components of this system have been defined, the interactions and interplay between them remain to be elucidated. Here, we investigated the interactions between Get3 and the Get4/Get5 complex (Get4/5) from Saccharomyces cerevisiae. We show that Get3 interacts with Get4/5 via an interface dominated by electrostatic forces. Using isothermal titration calorimetry and small-angle X-ray scattering, we further demonstrate that the Get3 homodimer interacts with two copies of the Get4/5 complex to form an extended conformation in solution.

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Acta Cryst. (2014). A70, C1740
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With rapid advances in the international scientific community as well as increasing demands for bright X-rays from users to facilitate their challenging scientific experiments, the construction of a new synchrotron facility was vital to maintaining National Synchrotron Radiation Research Center in Taiwan to be globally competitive. After conducting numerous assessments with our users, the decision to construct Taiwan Photon Source (TPS) was made at the meeting of Board of Trustee held in 2004 July. This large-scale project will establish, at the current campus of NSRRC, a new, low-emittance, synchrotron light source of circumference 518 m and with an electron beam of energy 3 GeV. TPS is designed to emphasize electron beams of small emittance and great brilliance for generating extremely bright photon beams. The superior characteristics of TPS have opened avenues for novel scientific opportunities and experimental techniques. The advanced techniques of phase-I beamlines include temporally coherent X-ray diffraction, protein microcrystollography, submicron soft X-ray spectroscopy, coherent X-ray scattering, submicron X-ray diffraction, X-ray nanoprobe, and high resolution inelastic soft X-ray scattering. Taking full advantage of the highly brilliant photon source, the seven planned beamline will aim for the forefront of science. These beamlines cover diverse research in physics, chemistry, biology, and material science, in the energy range from soft to hard X-rays for advanced research in spectroscopy, scattering and imaging.

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Acta Cryst. (2014). A70, C1747
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The coherent X-ray scattering beamline is one of the phase I beamlines designed for the Taiwan Photon Source, a new 3 GeV ring under construction at the National Synchrotron Radiation Research Center in Taiwan. By using a pair of 2m-long in-vacuum undulators, this beamline will provide a highly coherent beam for X-ray photon correlation spectroscopy principally; moreover, it will share a part of beamtime for small-angle X-ray scattering (SAXS) experiments with similar setup of the beamline. The operating photon energy is designed within the range of 5-20 keV. In vertical direction, the beam spot size at sample position is 1 μm with focusing mirror and by using 1D compound refractive lenses (CRLs) the beam spot size is 10 μm. The horizontal beam spot size is in the range of 1 to 10 μm with a two-stage focusing design. The vertical and horizontal transverse coherence lengths of the 10 μm2 beam spot size at the photon energy of 5 KeV are 212 and 6 μm at sample position respectively. Beside XPCS the beamline configuration can cope with the requirements of most SAXS experiments, including anomalous measurements and micro-beam mapping. In addition, the increasing biological SAXS demand is also considered and the on-line fast performance liquid chromatography (FPLC) will be enclosed for biological users.
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