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The influenza neuraminidase (NA) is a homotetramer with head, stalk, transmembrane and cytoplasmic regions. The structure of the NA head with a stalk has never been determined. The NA head from an N9 subtype influenza A virus, A/tern/Australia/G70C/1975 (H1N9), was expressed with an artificial stalk derived from the tetrabrachion (TB) tetramerization domain from Staphylo­thermus marinus. The NA was successfully crystallized both with and without the TB stalk, and the structures were determined to 2.6 and 2.3 Å resolution, respectively. Comparisons of the two NAs with the native N9 NA structure from egg-grown virus showed that the artificial TB stalk maintained the native NA head structure, supporting previous biological observations.

Supporting information

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Portable Document Format (PDF) file https://doi.org/10.1107/S2053230X18017892/va5018sup1.pdf
Supplementary Figures: protein purification and electron density of glycosylation chains at residues Asn75 (TB stalk) and Asn86 (N9).

PDB references: recombinant N9 with stalk, 6crd; recombinant N9 without stalk, 6mcx; N9 without stalk from egg-grown virus, 6d3b


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